Manalagi Apple Vinegar (Malus sylvestris Mill) as Anti Diabetic to Alloxan Induced Wistar White Male Rat

Abstract

Apple vinegar is estimated to be able to cure diabetes. Vinegar is able to
control the increase of glicemic index. Apple has antioxidant substance such as
quercetin and chlorogenic acid. Manalagi apple could be found easily in Indonesia.
This research aims to reveal the effect of Manalagi apple vinegar (Malus
sylvestris Mill) towards blood glucose level of alloxan induced Wistar white male rats
(Rattus norvegicus).
This research is experimental laboratory using Experimental methods. The
samples are 30 white rats divided into three groups for 31 days, 1) group which only
given standard diet; 2) group that induced by 120mg/dL alloxan; 3) group that
induced by 120mg/dL alloxan and Manalagi apple vinegar 0.27ml/100gram weight for
14 days. The measurement of blood serum glucose level is done on day twenty four
using homogenous colorimetric enzymatic test method for all groups.
Blood glucose average in group with only given standard diet as negative
control group (182.33 mg/dL), group which induced by 120mg/dL alloxan as positive
control group (205.2 mg/dL), group which induced by 120mg/dL alloxan and
Manalagi apple vinegar as treatment group (212.25 mg/dL). The result of One-Way
Anova significance is 0.229.
The conclusion of this research is that Manalagi apple vinegar treatment
doesn’t able to decrease the blood glucose level of white Wistar male rats
significantly.