PENGARUH PEMBERIAN EKSTRAK RUMPUT LAUT COKELAT (Sargassum duplicatum) TERHADAP PENINGKATAN KADAR HEMATOKRIT PADA DARAH TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus) ANEMIA YANG DI INDUKSI NaNO2
DOI:
https://doi.org/10.30649/obj.v3i2.47Keywords:
anemic, hematocrite, Sargassum duplicatum, NaNO2Abstract
Anemia is still a high prevalence disease in Indonesia, caused by a lack of iron in
the body. The components from brown seaweed such as cobalamin (vitamin B12),
pyridoxine (vitamin B6), chlorophyll and iron (Fe) can increase red blood cells in the body.
The purpose of this research was to determine the effect of brown seaweed (Sargassum
duplicatum) which can increase hematocrit levels in the blood of white male wistar strain
rat (Rattus norvegicus) suffered from anemia in induction NaNO2.
This research uses an experimental laboratory method with a post test only control
group design. The samples were 30 male rats divided into 3 treatment groups, the
negative control group (K0) without treatment, the positive control (K1) be treated anemia
given NaNO2 for 18 days without Sargassum duplicatum extract and treatment group (K2)
treated with anemia given NaNO2 for 18 days without Sargassum duplicatum extract at a
dose of 2,45gr / kgBB. On the 40th day, a blood sample was taken, then measured the
hematocrit level.
The results showed that the mean number of blood hematocrit levels in K2 was
higher than K1, it showed a significant difference descriptively. Based on the KruskalWallis test, a value of 0.260 was obtained that p > 0,05 showed that there were no
statistically significant differences between groups of experimental animals.
From the results of this research, the conclusion is the administration of brown
seaweed extract (Sargassum duplicatum) descriptively increased, but not statistically
significant.
References
Agawemu, C. S., Rumampuk, J. and Moningka, M. 2016, 'Hubungan Antara
Viskositas Darah Dengan Hematokrit pada Penderita Anemia dan Orang Normal',
Jurnal e-Biomedik, vol. 4, no. 1, p. 2.
Ambarsari, A. 2013, Analisis Model Matematika Pada Proses Produksi Sel Darah
(Hematopoiesis), skripsi, Fakultas Sains dan Teknologi, Universitas Islam
Negeri Maulana Malik Ibrahim Malang.
Arif, M. 2015, Penuntun Praktikum Hematologi, Fakultas Kedokteran Universitas
Hasanudin, Makassar.
Batubara, L., Kristina, T. N. and Rachmawati, B. 2016, ‘Effectiveness of Brown Algae
Extract to Reduce Serum Malondialdehyde and Protein Carbonyl Levels in Streptozotocin-Induced Sprague Dawley Rats’, Research Article, vol. 7, no.
, pp. 43–48.
Fitryadi, K. and Sutikno. 2013, ‘Pengenalan Jenis Golongan Darah Menggunakan
Jaringan Syaraf Tiruan Perceptron’, Jurnal Masyarakat Informatika, vol. 7, no.
, p. 2.
Ganong, W. F., 2015, Buku Ajar Fisiologi Kedokteran. Edisi 24. Jakarta, EGC.
Guyton, A. C., and Hall, J. E. 2016, Medical Physiology. Edisi 12. Jakarta, EGC.
Hall, J. E., 2010, Guyton and Hall Textbook of Medical Physiology. 12th edn, Edited
by Rebecca, G.J., Mississippi.
Hasanan, F. 2018, 'Hubungan Kadar Hemoglobin dengan Daya Tahan
Kardiovaskuler pada Atlet Atletik FIK Universitas Negeri Makassar',
Jurnal Olahraga Kesehatan, pp. 1–16.
Herawati, F. et al. 2011, 'Pedoman Interpretasi Data Klinik', Research Gate, p. 24-
Hutajulu, N. I., Taudjidi, A. A., and Fridaeyenti. 2015, ‘Gambaran Hematokrit pada
Pasien Stroke Iskemik di Rumah Sakit Umum Daerah Arifin Ahmad Provinsi
Riau’, JOM FK, vol. 2, no. 1, p. 2.
Indah E. et al. 2017, ‘Anemia pada Ibu Menyusui yang Bekerja’, Jurnal, vol. 1, pp.
–157.
Jumalang, F., Rotty, L., and Panda, A. 2015, 'Gambaran Kadar Hematokrit dan
Hemoglobin pada Kejadian Infark Miokard Akut di RSUP Prof. DR. R. D.
Kandou Manado Periode Januari-Agustus 2014', Jurnal e-Clinic, vol. 3, no. 1,
p. 283.
Kementrian Kesehatan Republik Indonesia. 2013, Riset Kesehatan Dasar.
Laihad, F. M., Sudiana, I. K., and Guritno, M. 2017, ‘Literature Review : Hyperbaric
Oxygen Theraphy on Mucormycosis Infection in Oral Cavity’, Folia Medica
Indonesiana, vol. 53, no. 2, p. 163.
Laila, I., 2017, 'Tinjauan Anemia Defisiensi Besi', Jurnal Kesehatan, vol. 1, no.1,
p. 7-26.
Lailani, M., Edward, Z., and Herman, R. B. 2013, ‘Gambaran Tekanan Darah Tikus
Wistar Jantan dan Betina Setelah Pemberian Diet Tinggi Garam’, Jurnal
Kesehatan Andalas, vol 2, no.3, p. 146-150.
Leets, I. et al. 2009, ‘Antioxidant Capacity, Polyphenol Content and Iron Bioavailability
From Algae (Ulva sp., Sargassum sp and Porphyra sp.) in Human Subjects',
British Journal of Nutrition, vol. 101, p. 79- 85.
Mardiah, A., Amir, A., Friadi, A., and Nasrul, E. 2018, 'Perbedaan Kadar Brain Derived
Neurotropic Factor', Jurnal Endurance, vol. 3, no. 3, p. 568-574.
Marinho, S. 2016, ‘Green Seaweed (Ulva lactuca dan Chaetomorpha crassa)',
Institut Pertanian Bogor.
Meenakshi, S. et al. 2009,‘Total Flavanoid and in vitro Antioxidant Activity of Two
Seaweeds of Rameshwaram Coast’, Global Journal of Pharmacology, vol.
, no. 2, pp. 59–62.
Mehta, A., and Hoffbrand, A. 2010, Haematology at a Glance, Jakarta, EMS Press
Munawaroh, S. 2009, Pengaruh Ekstrak Kelopak Rosela (Hibiscus sabdariffa)
Terhadap Peningkatan Jumlah Eritrosit dan Kadar Hemoglobin (Hb) Dalam
Darah Tikus Putih (Rattus norvegicus) Anemia, Skripsi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Maulana Malik Ibrahim Malang.
Mallo, P. et al. 2012, 'Rancang Bangun Alat Ukur Kadar Hemoglobin dan Oksigen
Dalam Darah dengan Sensor Oximeter Secara Non- Invasive', Jurnal, vol. 1,
no.1.
Pohuwato, P. et al. 2018, ‘Karakteristik Rumput Laut Cokelat Characteristics of
Brown Seaweeds Sargassum policystum and Padina minor from Pohuwato
Water', Jurnal, p 21.
Putranti, R. 2013, ‘Skrining Fitokimia dan Aktivitas Antioksidan Ekstrak Rumput Laut
Sargassum duplicatum dan Turbinaria ornata’. Jurnal Kesehatan, vol. 1, no.1.
Rahmi, R. and Nazro, Z. 2018, ‘Efektifitas Konsumsi Rumput Laut Untuk
Meningkatkan Kadar Haemoglobin Pada Ibu Hamil Anemia’, Jurnal
Endurance, vol. 3, no. 1, p. 195–199.
Sari, I. 2016, 'Tinjauan Tentang Darah', Jurnal Kesehatan, vol. 3, no. 1, p. 2-29.
Suryani, D., Hafiani, R., and Junita, R. 2015. 'Analisis Pola Makan dan Anemia Gizi
Besi pada Remaja Putri Kota Bengkulu', Jurnal Kesehatan Masyarakat
Andalas, vol. 10, no. 1, pp. 11–18.
Susiloningtyas, Is. 2019. 'Pemberian Zat Besi (Fe) dalam Kehamilan', Jurnal
Unissula.
Salam, S. W., 2012. Gambaran Jumlah Sel Darah Merah, Kadar Hemoglobin, Kadar
Hematokrit, dan Indeks Eritrosit pada Kerbau Lumpur (Bubalus bubalis) Betina,
Skripsi, Institut Pertanian Bogor.
Santika, M., Trisnawati, P., and Artawan, I. 2014. ‘Studi Pengaruh Lamanya
Pemaparan Medan Magnet Terhadap Jumlah Sel Darah Putih (Leukosit)
pada Tikus Putih (Rattus norvegicus)’, Jurnal Buletin Fisika, vol. 15, no. 1, pp.
–38.
Setiawan, A., Suryani, E., and Kunci, K. 2014. ‘Segmentasi Citra Sel Darah Merah
Berdasarkan Morfologi Sel Untuk Mendeteksi Anemia Defisiensi Besi’,
Jurnal Itsmart, vol. 3, no. 1, pp. 1–8.
Simamora, D., Kartasurya, M. I., and Pradigdo, S. F. 2018. ‘Tentang Rumput Laut
Cokelat’, Jurnal Keanekaragaman Budidaya, vol. 1, no. 1, p 6.
Simarmata, R. R., and Wheny, N. 2017. ‘Aktivitas Jus Buah Terong Belanda
terhadap Kadar Hemoglobin’, Jurnal Biologi, vol. 6, no. 2, pp. 69–74.
Spontan, P., and Padang, M. D. 2013, ‘Artikel Penelitian Hubungan Jumlah
Trombosit dengan Nilai Hematokrit pada Penderita Demam Berdarah Dengue
dengan Manifestasi’, Jurnal Kesehatan, vol. 6, no. 2, pp. 446–451.
Suparmi and Achmad, S. 2009, ‘Mengenal Potensi Rumput Laut : Kajian Pemanfaatan
Sumber Daya Rumput Laut Dari Aspek Industri Dan Kesehatan’, Jurnal Sultan
Agung, vol. XLIV, no. 118, pp. 95–116.
Widyastuti, D. A. et al. 2013, ‘Profil Darah Tikus Putih Wistar pada Kondisi Subkronis
Pemberian Natrium Nitrit’, Jurnal Kesehatan, vol. 31, no. 2, pp. 201–215.
Wulandari, A. 2017, ‘Keanekaragaman Morfologi Rumput Laut Sargassum dari
Pantai’, Jurnal Pengembangan Sumber Daya, pp. 6–24.
Yuniarti, A., and Hadisaputro, S. 2017, ‘Pengaruh Pemberian Rumput Laut
Sargassum sp Terhadap Kadar Hemoglobin dan Feritin Serum’, Jurnal
Gizi Indonesia, vol. 5, no. 1, p. 7.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Oceana Biomedicina Journal
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
