Analisa in Silico Kunyit (Curcuma longa) sebagai Inhibitor Murine Double Minute 2 Protein untuk Terapi Glioblastoma Multiforme
DOI:
https://doi.org/10.30649/obj.v3i2.61Kata Kunci:
Glioblastoma multiforme, Curcuma longa, MDM 2, Autodock 4.2Abstrak
Tumor otak meliputi berbagai kanker yang tumbuh dari sel otak (tumor otak primer)
ataupun berasal dari tumor sistemik yang mengalami metastasis ke otak (tumor otak
sekunder). Dari seluruh tipe tumor otak primer, Glioblastoma Multiforme merupakan
tumor otak yang paling sering dijumpai dan merupakan salah satu yang paling ganas.
Pada 85% kasus Glioblastoma Multiforme, umumnya ditemukan kaitan dengan adanya
gangguan tingkat molekuler pada jalur tumor suppresor gene p53, sehingga semakin
banyak terapi yang dikembangkan dengan berfokus pada jalur ini. Salah satu jalur yang
dapat dipakai sebagai model terapi adalah menginhibisi protein murine double minute 2
yang merupakan inhibitor dari p53. Kunyit (curcuma longa) adalah salah satu tanamantradisional yang sudah sangat sering digunakan dalam dunia medis dan berbagai ekstrak
nya telah diteliti mempunyai efek anti-kanker.
Penelitian ini adalah sebuah studi in silico yang meneliti potensi berbagai bahan
kimia aktif dari kunyit sebagai inhibitor pada protein murine double minute 2
menggunakan AutoDock 4.2 dan berdasarkan prinsip algoritma genetik Lamarckian.
Hasil docking menunjukkan binding energy berkisar dari rentang -4.81 kcal/mol sampai -
2.34 kcal/mol, dengan senyawa curcumenol mempunyai binding energy yang paling kecil
dan curcumin mempunyai binding energy yang paling besar. Studi ini dapat digunakan
sebagai dasar untuk melakukan penelitian lebih lanjut (in vivo dan in vitro) terkait bahan
kimia aktif kunyit dan efek nya sebagai terapi Glioblastoma Multiforme..
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