The infection of Vibrio parahaemolyticus in shrimp and human

Rian Ka Praja


Vibrio parahaemolyticus is an aquatic zoonotic agent that can threaten human and aquaculture animal health. Humans can be infected by consuming contaminated raw seafood or wound-related infections. Generally infection of V. parahemolyticus is orally transmitted and causes gastroenteritis in humans while in aquaculture animals especially shrimp can cause Acute Hepatopancreatic Necrosis Disease (AHPND) or Early Mortality Syndrome (EMS) with a very high mortality rate and cause economic losses. Shrimp species susceptible to infection are Litopenaeus vannamei, Penaeus monodon, and P. chinensis. V. parahaemolyticus produces several toxins in human disease such as thermostable direct hemolysin (TDH), TDH-related haemolysin (TRH), and thermolabile hemolysin (TLH). Meanwhile, Photorabdus insect-related (Pir) toxins consisting of PirAvp and PirBvp are the toxins associated with AHPND in shrimp. The genes that encode the toxin are used as targets to diagnose V. parahaemolyticus pathogens molecularly. Until now the treatment of V. parahaemolyticus infection is using antibiotics and fluid therapy, but there were V. parahaemolyticus isolates from aquaculture that have been resistant to antibiotics so that the use of antibiotics in aquaculture must be controlled and the use of alternative therapy are very important to be developed to control V. parahaemolyticus infection.


Keywords: V. parahaemolyticus, zoonotic, gastroenteritis, Acute Hepatopancreatic Necrosis Disease (AHPND), Early Mortality Syndrome (EMS).

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Abd-Elghany, S.M. and Sallam, K.I. 2013. Occurrence and molecular identification of Vibrio parahaemolyticus in retail shellfish in Mansoura, Egypt. Food Control. 33: 399-405.

Alagappan, K.M., Deivasigamani, D., Somasundaram, S.T. and Kumaran, S. 2010. Occurrence of Vibrio parahaemolyticus and Its Specific Phages from Shrimp Ponds in East Coast of India. Curr Microbiol. 61(4):235-40.

Andrews, L.S., Park, D.L. and Chen, Y.P. 2000. Low temperature pasteurization to reduce the risk of Vibrio infections from raw shell-stock oysters. Food Addit. Contam.17:787-791.

Anjay, Das, S.C., Kumar, A., Kaushik, P. and Kurmi, B. 2014. Occurrence of Vibrio parahaemolyiticus in marine fish and sellfish. Indian Journal of Geo-Marine Sciences. 43(5): 887-890.

Austin, B. 2010. Vibrios as causal agents of zoonoses. Veterinary Microbiology. 140(3-4): 1-34.

Broberg, C.A., Calder, T.J., Orth, K. 2011. Vibrio parahaemolyticus cell biology and pathogenicity determinants. Microbes Infect. 13(12-13): 992-1001.

Brooks, G.F., Carrol, K.C., Butel, J.S., Morse, S.A. and Mietzner, T.A. 2013. Medical Microbiology 26th Edition. McGraw-Hill Companies Inc.

Caburlotto, G., Gennari, M., Ghidini, V., Tafi, M.C. and Lleo, M.M. 2010. Serological and molecular characterization of Vibrio parahaemolyticus marine strains carrying pandemic genetic markers. The ISME Journal. 4: 1071–1074.

Ceccarelli, D., Hasan, N.A., Huq, A. and Colwell, R.R. 2013. Distribution and dynamics of epidemic and pandemic Vibrio parahaemolyticus virulence factors. Front Cell Infect Microbiol. 3(97): 1-9.

Changchai, N. and Saunjit, S. 2014. Occurrence of Vibrio parahaemolyticus and Vibrio vulnificus in retail raw oysters from the eastern coast of Thailand. Southeast Asian J. Trop. Med. Public Health. 45: 662–669.

Chao, G., Jiao, X., Zhou, X., Yang, Z., Huang, J., Pan, Z., Zhou, L. and Qian, X. 2009. Serodiversity, pandemic O3:K6 clone, molecular typing, and antibiotic susceptibility of foodborne and clinical Vibrio parahaemolyticus isolates in Jiangsu, China. Foodborne Pathog Dis. 6(8):1021-8.

Cho, S.H., Shin, H.H., Choi, Y.H., Park, M.S. and Lee, B.K. 2008. Enteric bacteria isolated from acute diarrheal patients in the Republic of Korea between the year 2004 and 2006. J. Microbiol. 46: 325-330.

Chu, K. B., Ahmad, I., Siti Zahrah, A., Irene, J., Norazila, J., Nik Haiha, N. and Teoh, T. P. 2016. Current status of acute hepatopancreatic necrosis disease (AHPND) of farmed shrimp in Malaysia. Proceedings of the ASEAN Regional Technical Consultation on EMS/AHPND and Other Transboundary Diseases for Improved Aquatic Animal Health in Southeast Asia, 22-24 February 2016, Makati City, Philippines (pp. 55-59). Tigbauan, Iloilo,

Croci, L., Suffredini, E., Cozzi, L. and Toti, L. 2002. Effects of depuration of molluscs experimentally contaminated with Escherichia coli, Vibrio cholerae O1 and Vibrio parahaemolyticus. J. Appl. Microbiol. 92:460-465.

De Schryver, P., Defoirdt, T. and Sorgeloos, P. 2014. Early Mortality Syndrome Outbreaks: A Microbial Management Issue in Shrimp Farming? PLoS Pathog. 10(4): e1003919.

DePaola, A., Kaysner, C.A., Bowers, J. and Cook, D. W. 2000. Environmental investigations of Vibrio parahaemolyticus in oysters after outbreaks in Washington, Texas, and New York (1997 and 1998). Appl. Environ. Microbiol. 66: 4649–4654.

Drake, S.L., DePaola, A. and Jaykus L.A. 2007. An overview of Vibrio vulnificus and Vibrio parahaemolyticus. Compr Rev Food Sci Food Saf. 6:120–144.

Fabbro, C., Cataletto, B. and Del Negro, P. 2010. Detection of pathogenic Vibrio parahaemolyticus through biochemical and molecular-based methodologies in coastal waters of the Gulf of Trieste (North Adriatic Sea). FEMS Microbiol Lett. 307:158-64.

Feldhusen, F. 2000. The role of seafood in bacterial food borne diseases. Microbes and Infection. 2: 1651-1660.

Flegel, T.W. 2012. Historic emergence, impact and current status of shrimp pathogens in Asia. J. Invert. Pathol. 110: 166-173.

Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO). 2011. Risk assessment of Vibrio parahaemolyticus in seafood: Interpretative summary and Technical report. Microbiological Risk Assessment Series No. 16. Rome.

Gomez-Gil, B., Soto-Rodríguez, S., Lozano, R. and Betancourt-Lozano, M. 2014. Draft genome sequence of Vibrio parahaemolyticus Strain M0605, which causes severe mortalities of shrimps in Mexico. Genome Announc. 2(2): e00055-14.

Ham, H. and Orth, K. 2012. The role of type III secretion system 2 in Vibrio parahaemolyticus pathogenicity. J Microbiol. 50:719-25.

Hara-Kudo, Y., Sugiyama, K., Nishibuchi, M., Chowdhury, A., Jun Yatsuyanagi, J., Ohtomo, Y., Saito, A., Nagano, N., Nishina, T., Nakagawa, H., Konuma, H., Miyahara, M. and Kumagai, S. 2003. Prevalence of pandemic thermostable direct haemolysin producing Vibrio parahaemolyticus O3:K6 in seafood and the coastal environment in Japan. Applied and Environmental Microbiology. 69:3883-3891.

Hiyoshi, H., Kodama, T., Iida, T. and Honda T. 2010. Contribution of Vibrio parahaemolyticus Virulence Factors to Cytotoxicity, Enterotoxicity, and Lethality in Mice. Infect. Immun. 78(4): 1772-1780.

Honda, T., Iida, T., Akeda, Y. and Kodame, T. 2008. Sixty years of Vibrio parahaemolyticus research. Microbe. 3: 462–466.

Hornstrup, M.K. and Gahrnhansen, B. 1993. Extraintestinal infections caused by Vibrio parahaemolyticus and Vibrio alginolyticus in a Danish county, 1987-1992. Scand. J. Infect. Dis. 25: 735-740.

Johnston, M.D. and Brown, M.H. 2002. An investigation into the changed physiological state of Vibrio bacteria as a survival mechanism in response to cold temperatures and studies on their sensitivity to heating and freezing. J Appl Microbiol. 92(6):1066-77.

Jones, J.L., Ludeke, C.H., Bowers, J.C., Garrett, N., Fischer, M., Parsons, M.B., Bopp, C.A. and DePaola, A. 2012. Biochemical, serological, and virulence characterization of clinical and oyster Vibrio parahaemolyticus isolates. J. Clin. Microbiol. 50(7): 2343–2352.

Kanjanasopa, D., Pimpa, B., and Chowpongpang S. 2011. Occurrence of Vibrio parahaemolyticus in cockle (Anadara granosa) harvested from the south coast of Thailand. Songklanakarin J. Sci. Technol. 33 (3): 295-300.

Khimmakthong, U. and Sukkarun, P. 2017. The spread of Vibrio parahaemolyticus in tissues of the Pacific white shrimp Litopenaeus vannamei analyzed by PCR and histopathology. Microb Pathog. 113:107-112.

Kim, Y.B., Okuda, J., Matsumoto, C., Takahashi, N., Hashimoto, S. and Nishibuchi, M. 1999. Identification of Vibrio parahaemolyticus strains at the species level by PCR targeted to the toxR gene. J Clin Microbiol. 37:1173–1177.

Lai, H.C., Ng, T.H., Ando, M., Lee, C.T., Chen, I.T., Chuang, J.C., Mavichak, R., Chang, S.H., Yeh, M.D., Chiang, Y.A., Takeyama, H., Hamaguchi, H.O., Lo, C.F., Aoki, T. and Wang, H.C. 2015. Pathogenesis of acute hepatopancreatic necrosis disease (AHPND) in shrimp. Fish Shellfish Immunol. 47(2):1006-14.

Lee, C.T., Chen, I., Yang, Y., Ko, T., Huang, Y., Huang, J., Lin, S., Chen, C., Lin, S., Lightner, D.V., Wang, H.C., Huang, F., Wang, A.H.J., Wang, H., Hor, L. and Lo, C. 2015. The opportunistic marine pathogen Vibrio parahaemolyticus becomes virulent by acquiring a plasmid that expresses a deadly toxin. Proc Natl Acad Sci U S A. 112(34):10798-803.

Li, P., Kinch, L.N., Ray, A., Dalia, A.B., Cong, Q., Nunan, L.M., Camilli, A., Grishin, N.V., Salomon, D. and Orth, K. 2017. Acute Hepatopancreatic Necrosis Disease-Causing Vibrio parahaemolyticus Strains Maintain an Antibacterial Type VI Secretion System with Versatile Effector Repertoires. Appl Environ Microbiol. 16; 83(13). pii: e00737-17.

Lin, Z., Kumagai, K., Baba, K., Mekalanos, J.J. and Nishibuchi, M. 1993. Vibrio parahaemolyticus has a homolog of the Vibrio cholera toxRS operon that mediates environmentally induced regulation of the thermostable direct hemolysin gene. J. Bacteriol. 175: 3844-385.

Lomelí-Ortega C.O. and Martínez-Díaz S.F. 2014. Phage therapy against Vibrio parahaemolyticus infection in the whiteleg shrimp (Litopenaeus vannamei) larvae. Aquaculture. 434: 208–211.

Lozano-León, A., Torres, J., Osorio, C.R. and Martínez-Urtaza, J. 2003. Identification of tdh-positive Vibrio parahaemolyticus from an outbreak associated with raw oyster consumption in Spain. FEMS Microbiol. Lett. 226: 281–284.

Marlina, Radu, S., Kqueen, C.Y., Napis, S., Zakaria, Z., Mutalib, S.A. and Nishibuchi, M. 2007. Detection of tdh and trh genes in Vibrio parahaemolyticus isolated from Corbicula moltkiana prime in west sumatera, Indonesia. Southeast Asian J Trop Med Public Health. 38(2): 349-355.

Nair, G.B, Ramamurthy, T., Bhattacharya, S.K., Dutta, B., Takeda, Y. and Sack, D.A. 2007. Global dissemination of Vibrio parahaemolyticus serotype O3:K6 and its serovariants. Clin. Microbiol. Rev. 20: 39–48.

Nelapati, S. and Krishnaiah, N. 2010. Detection of total and pathogenic Vibrio parahaemolyticus by Polymerase Chain Reaction using toxR, tdh and trh genes. Veterinary World. 3(6): 268-271.

Nelapati, S., Nelapati, K. and Chinnam, B.K. 2012. Vibrio parahaemolyticus - An emerging foodborne pathogen – A Review. Veterinary World. 5(1): 48-62.

Oh, E.G., Son, K.T., Yu, H., Lee, T.E., Lee, H.J., Shin, S., Kwon, J.Y., Park, K. and Kim, J. 2011. Antimicrobial resistance of Vibrio parahaemolyticus and Vibrio alginolyticus Strains Isolated from Farmed Fish in Korea from 2005 through 2007. J. Food. Protect. 74(3): 380-386.

Pattukumar, V., Sahu, M.K., Sethubathi, G.V., Murugan, M., Sivakumar, K. and Arul, V. 2010. Population of Vibrio Parahaemolyticus (Pathogen) and Bacillus (Beneficial Bacteria) in Penaeus Monodon (Fabricus, 1798) Culture. OnLine Journal of Biological Sciences. 10 (4): 142-150.

Pazhani, G.P., Bhowmik, S.K., Ghosh, S., Guin, S., Dutta, S., Rajendran, K., Saha, D.R., Nandy, R.K., Bhattacharya, M.K., Mukhopadhyay, A.K. and Ramamurthy, T. 2014. Trends in the epidemiology of pandemic and non-pandemic strains of Vibrio parahaemolyticus isolated from diarrheal patients in Kolkata, India. PLoS Negl Trop Dis. 8(5): e2815.

Pui, C.F., Bilung, L.M., Zin, N.B.M., Abidin, N.N.B.Z., Vincent, M. and Apun, K. 2014. Risk of Acquiring Vibrio parahaemolyticus in Water and Shrimp from an Aquaculture Farm. Kuroshio Science.8(1): 59-62.

Qadri, F., M.S. Alam, M. Nishibuchi, Rahman, T., Alam, N.H., Chisti, J., Kondo, S., Sugiyama, J., Bhuiyan, N.A., Mathan, M.M., Sack, D.A. and Nair, G.B. 2003. Adaptive and inflammatory immune responses in patients infected with strains of Vibrio parahaemolyticus. J. Infect. Dis. 187: 1085-1096.

Raghunath, P. 2014. Roles of thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) in Vibrio parahaemolyticus. Front Microbiol. 5(805): 1-4.

Rahimi, E., Ameri, M., Doosti, A, and Gholampour, A.R. 2010. Occurrence of toxigenic Vibrio parahaemolyticus strains in shrimp in Iran. Foodborne Pathog. Dis. 7: 1107–1111.

Ren, T. and Su, Y.C. 2006. Effects of electrolyzed oxidizing water treatment on reducing Vibrio parahaemolyticus and Vibrio vulnificus in raw oysters. J. Food Prot.69: 1829–1834.

Shimohata, T. and Takahashi, A. 2010. Diarrhea induced by infection of Vibrio parahaemolyticus. J Med Invest. 57:179-82.

Soto-Rodriguez, S.A., Gomez-Gil, B., Lozano-Olvera, R., Betancourt-Lozano, M. and Morales-Covarrubias, M.S. 2015. Field and experimental evidence of Vibrio parahaemolyticus as the causative agent of acute hepatopancreatic necrosis disease of cultured shrimp (Litopenaeus vannamei) in northwestern Mexico. Appl Environ Microbiol. 81:1689 –1699.

Stones, D.H. and Krachler, A.M. 2015. Dual function of a bacterial protein as an adhesin and extracellular effector of host GTPase signaling. Small GTPases. 6:153-6.

Su, Y.C. and Liu, C.C. 2007. Vibrio parahaemolyticus: a concern of seafood safety. Food Microbiol. 24(6):549-58.

Teplitski, M., Wright, A.C. and Lorca, G. 2009. Biological approaches for controlling shellfish-associated pathogens. Current Opinion in Biotechnology. 20(2): 185-190.

Tinwongger, S., Proespraiwong, P., Thawonsuwan, J., Sriwanayos, P., Kongkumnerd, J., Chaweepack, T., Mavichak, R., Unajak, S., Nozaki, R., Kondo, H. and Hirono, K. 2014. Development of PCR Diagnosis for Shrimp Acute Hepatopancreatic Necrosis Disease (AHPND) Strain of Vibrio parahaemolyticus. Fish Pathology. 49(4): 159-164.

Venkateswaran, K., Dohmoto, N. and Harayama, S. 1998. Cloning and Nucleotide Sequence of the gyrB Gene of Vibrio parahaemolyticus and Its Application in Detection of This Pathogen in Shrimp. Appl Environ Microbiol. 64(2): 681–687.

Wang, R., Huang, J., Zhang, W., Lin, G., Lian, J., Jiang, L., Lin, H., Wang, S. and Wang, S. 2011. Detection and identification of Vibrio parahaemolyticus by multiplex PCR and DNA-DNA hybridization on a microarray. J. Genet. Genomics 38: 129–135.

Wang, R., Zhong, Y., Gu, X., Yuan, J., Saeed, A.F. and Wang, S. 2015. The pathogenesis, detection, and prevention of Vibrio parahaemolyticus. Front. Microbiol. 6:144.

Wang, Y., Li, D., Wang, Y., Li, K. and Ye, C. 2016. Rapid and Sensitive Detection of Vibrio parahaemolyticus and Vibrio vulnificus by Multiple Endonuclease Restriction Real-Time Loop-Mediated Isothermal Amplification Technique. Molecules. 21(111): 1-16.

Waterfield, N., Kamita, S.G., Hammock, B.D. and Ffrench-Constant, R. 2005. The Photorhabdus Pir toxins are similar to a developmentally regulated insect protein but show no juvenile hormone esterase activity. FEMS Microbiol Lett. 245:47–52.

Wong, H.C., Liu, S.H., Wang, T.K., Lee, C.L., Chiou, C.S., Liu, D.P., Nishibuchi, M. and Lee, B.K. 2000. Characterization of Vibrio parahaemolyticus O3:K6 from Asia. Applied and Environmental Microbiology. 66: 3981-3986.

Xu, X., Cheng, J., Wu, Q., Zhang, J. and Xie, T. 2016. Prevalence, characterization, and antibiotic susceptibility of Vibrio parahaemolyticus isolated from retail aquatic products in North China. BMC Microbiology. 16(32): 1-9.

Xu, X.K., Wu, Q.P., Zhang, J.M., Cheng, J.H., Zhang, S.H. and Wu, K. 2014. Prevalence, pathogenicity, and serotypes of Vibrio parahaemolyticus in shrimp from Chinese retail markets. Food Control. 46: 81–85.

Yamazaki, W., Kumeda, Y., Uemura, R. and Misawa, N. 2011. Evaluation of a loop-mediated isothermal amplification assay for rapid and simple detection of Vibrio parahaemolyticus in naturally contaminated seafood samples. Food Microbiol. 28: 1238–1241.

Yeung, P.S. and Boor, K.J. 2004. Epidemiology, pathogenesis, and prevention of foodborne Vibrio parahaemolyticus infections. Foodborne Pathog. Dis. 1: 74–88. doi: 10.1089/153531404323143594

Zhang, Q., Dong, X., Chen, B., Zhang, Y., Li, W. and Zu, Y. 2016. Zebrafish as a useful model for zoonotic Vibrio parahaemolyticus pathogenicity in fish and human. Developmental and Comparative Immunology. 55: 159-168.



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