The Effect of Jeruju Leaf Extract (Acanthus ilicifolius) on Decrease Blood Level of LDL of Male Wistar Rat (Rattus norvegicus) Induced Alloxan

Anggita Saraswati, Irmawati M. Dikman, Retno Budiarti

Abstract


ABSTRACT

Background : DM is a chronic disease that has long-term complications. Alloxan is a fast way to produce experimental diabetic (hyperglycemic) in animal experiments related to elevated LDL. Jeruju (Acanthus ilicifolius) is a low-growth herbaceous plant that easily grows containing secondary metabolites such as Alkaloids, Saponins, Flavonoids, Terpenoids, and Phenols.

Objective : To determine the effect of jeruju leaf extract on the decrease in blood levels of LDL of male Wistar rat (Rattus norvegicus) induced Alloxan.

Methods : This research is an experimental laboratory type with Post Test Only Control Group Design. The sample using 24 rats, which were divided into 3 groups: negative control group (K-) were given standard feed; positive control group (K +) induced alloxan 120 mg/kgBW on 1st day; the treatment group (P) induced alloxan 120 mg/kgBW on 1st day and was given jeruju leaf extract 67.2 mg/kgBW on 5th-14th day. The 15th day a blood sample was taken to determine LDL levels.

Result : The results of One-Way ANOVA test showed an insignificant mean difference (p = 0.270) in LDL level of K- ( = 9.75 mg/dl) and the K+ ( = 11.38 mg/dl ). Insignificant mean difference (p = 0.270) in P ( = 9 mg/dl) with blood LDL levels in the K+ ( = 11.38 mg/dl) and the K- ( = 9, 75 mg/dl).

Conclusion :    Giving alloxan can increase blood levels of LDL and administration of jeruju leaf extract can reduce blood levels of LDL in experimental animals but not statistically significant.

 

Keywords: Alloxan, LDL, Acanthus ilicifolius.

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DOI: http://dx.doi.org/10.30649/obj.v3i2.50

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