Comparison of Cerebral Malaria Mice Models Infected by P. berghei ANKA

Prawesty Diah Utami, R. Varidianto Yudo Tjahjono, Indi Jazilah, Ismy Azizah Sisnawati

Abstract


The high morbidity and mortality due to malaria infection makes it a global health problem. The pathogenesis and pathophysiology of cerebral malaria has not been fully understood. The use of cerebral malaria model mice is one alternative to understand its pathogenesis and pathophysiology. The purpose of this study was to compare Swiss mice and C57BL / 6 mice inoculated by P.berghei ANKA as a model of cerebral malaria mice. Methods: The study design was a randomized Post Test Control Only Group Design using 12 Swiss mice and 12 C57BL / 6 mice divided into 2 groups each: the treatment group inoculated by P. berghei ANKA and the control group without inoculation. Observation of clinical manifestations and parasitemia level was carried out from day 1 to day -10. The study results showed that K2 group showed a significant increase in the degree of parasitemia compared to K4. K4 group showed clinical manifestations of cerebral malaria while K2 did not show clinical manifestations of cerebral malaria. Conclusion: C57BL / 6 mice inoculated by PbA can be used as cerebral malaria animal models.

 

Keywords: P.berghei ANKA, C57BL/6 mice, Swiss mice, Clinical manifestation, Parasitemia level.


Full Text:

PDF

References


Adetutu, Adewale, Olubukola S. Olorunnisola, Abiodun O. Owoade, and Peter Adegbola. 2016. “Inhibition of In Vivo Growth of Plasmodium Berghei by Launaea Taraxacifolia and Amaranthus Viridis in Mice.” Malaria Research and Treatment 2016 (1): 1–9. https://doi.org/10.1155/2016/9248024.

Baeti, Devi Nurul. 2010. Efek Terapi Kombinasi Klorokuin Dan Serbuk Lumbricus Rubellus Terhadap Ekspresi Gen ICAM 1 Pada Mencit Swiss Yang Diinfeksi P. Berghei ANKA. Fakultas Kedokteran Sebelas Maret.

Blanco Yara , Farias Alessandro, Goelnitz Uta, Amino Rogério Lopes Stefanie , Arrais-Silva Wagner , Carvalho Bruna, and Costa Fabio Wunderlich Gerhard, Santos Leonilda, Giorgio Selma. 2008. “Hyperbaric Oxygen Prevents Early Death Caused by Experimental Cerebral Malaria.” PLoS ONE 3 (9): 2–11. https://doi.org/10.1371/journal.pone.0003126.

Doolan, Denise L. 2002. Methods and Protocols Edited By. Humana Press Inc. Totowa.

Einstein, Medicine College of Albert. 2015. “Recommended Methods of Anesthesia , Analgesia , and Euthanasia for Laboratory Animal Species.” In Laboratory Animal, 1:1–12.

Evans, Sharon S, Elizabeth A Repasky, and Daniel T Fisher. 2016. “Fever and the Thermal Regulation of Immunity: The Immune System Feels the Heat.” Nature Reviews Immunology 15 (6): 335–49. https://doi.org/10.1038/nri3843.Fever.

Franke-fayard, Blandine, Jannik Fonager, Anneke Braks, Shahid M Khan, and Chris J Janse. 2010. “Sequestration and Tissue Accumulation of Human Malaria Parasites : Can We Learn Anything from Rodent Models of Malaria ? Sequestration and Tissue Accumulation of Human Malaria Parasites : Can We Learn Anything from Rodent Models of Malaria ?,” no. June 2017. https://doi.org/10.1371/journal.ppat.1001032.

Franke-fayard, Blandine, Chris J Janse, Cunha R. Margarida, Jai Ramesar, Philippe Buscher, Ivo Que, Clemens Lowik, et al. 2005. “Murine Malaria Parasite Sequestration : CD36 Is the Major Receptor , but Cerebral Pathology Is Unlinked.”

Janse, Chris. 2016. “P.Berghei.” Netherlands: Leids Universitairs Medisch Centrum.

Martins, Yuri Chaves, Mary Jane Smith, Marcelo Pelajo-Machado, Guilherme Loureiro Werneck, Henrique Leonel Lenzi, Claudio Tadeu Daniel-Ribeiro, and Leonardo José De Moura Carvalho. 2009. “Characterization of Cerebral Malaria in the Outbred Swiss Webster Mouse Infected by Plasmodium Berghei ANKA.” International Journal of Experimental Pathology 90 (2): 119–30. https://doi.org/10.1111/j.1365-2613.2008.00622.x.

Muntiha, Mohamad. 2001. Teknik Pembuatan Preparat Dengan Pewarnaan Hematoksilin Dan. Balai Penelitian Veteriner.

Nurhayati. 2009. “Status Hematologi Penderita.” Majalah Kedokteran Andalas 33 (1): 48–53.

Oakley, Miranda S., Thomas F. McCutchan, Vivek Anantharaman, Jerrold M. Ward, Laurence Faucette, Cindy Erexson, Babita Mahajan, et al. 2008. “Host Biomarkers and Biological Pathways That Are Associated with the Expression of Experimental Cerebral Malaria in Mice.” Infection and Immunity 76 (10): 4518–29. https://doi.org/10.1128/IAI.00525-08.

Rahmad, Darmawan. 2014. “Uji Aktivitas Antiplasmodium Ekstrak Daun Sungkai ( Peronema Canescens ) Terhadap Mencit Jantan ( Mus Musculus ) Serta Implementasinya Sebagai LKS Pada Materi Protista.” Universitas Bengkulu.

Runtuk, Kresna Septiandy, Loeki Enggar Fitri, and Rintis Noviyanti. 2018. “Role of Hypoxia-Inducible Factor (HIF)-1α and CD11b in Pathogenesis of Experimental Cerebral Malaria in Mice.” Acta Microbiologica Hellenica 63 (01): 65–72.

Sierro, Frederic, and Georges E.R. Grau. 2019. “The Ins and Outs of Cerebral Malaria Pathogenesis: Immunopathology, Extracellular Vesicles, Immunometabolism, and Trained Immunity.” Frontiers in Immunology 10 (830): 1–11. https://doi.org/10.3389/fimmu.2019.00830.

Souza, J Brian D E, Julius C R Hafalla, and Eleanor M Riley. 2009. “Cerebral Malaria : Why Experimental Murine Models Are Required to Understand the Pathogenesis of Disease” 137. https://doi.org/10.1017/S0031182009991715.

Storm, Janet, and Alister Craig. 2014. “Pathogenesis of Cerebral Malaria--Inflammation and Cytoadherence.” Frontiers in Cellular and Infection Microbiology 4 (July): 100. https://doi.org/10.3389/fcimb.2014.00100.

Waknine-Grinberg, Judith H., Nicholas Hunt, Annael Bentura-Marciano, James A. McQuillan, Ho Wai Chan, Wing Chi Chan, Yechezkel Barenholz, Richard K. Haynes, and Jacob Golenser. 2010. “Artemisone Effective against Murine Cerebral Malaria.” Malaria Journal 9 (1): 1–15. https://doi.org/10.1186/1475-2875-9-227.




DOI: http://dx.doi.org/10.30649/obj.v3i2.49

Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Prawesty Diah Utami

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

 

Translation: https://www.onlinedoctranslator.com

Duplication checking by: https://www.duplichecker.com

Plagiarism detection by: https://plagiarismdetector.net

 Zotero

Published by: Universitas Hang Tuah, Surabaya, Jawa Timur, Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.